Decarboxylation of α-difluoromethylornithine by ornithine decarboxylase
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چکیده
منابع مشابه
Decarboxylation of a - difluoromethylornithine by ornithine decarboxylase Anthony
The mechanism of inactivation of rodent ornithine decarboxylase by a-difluoromethylornithine (DFMO) was studied using the inhibitor labelled with 14C in both the 1 and the 5 positions. [1-14C]DFMO was a substrate and was decarboxylated by the enzyme yielding '4CO2. A radioactive metabolite derived from [5-'4C]DFMO was bound to the enzyme, and the extent of binding paralleled the irreversible in...
متن کاملInhibition of human ornithine decarboxylase activity by enantiomers of difluoromethylornithine.
Racemic difluoromethylornithine (D/L-DFMO) is an inhibitor of ODC (ornithine decarboxylase), the first enzyme in eukaryotic polyamine biosynthesis. D/L-DFMO is an effective anti-parasitic agent and inhibitor of mammalian cell growth and development. Purified human ODC-catalysed ornithine decarboxylation is highly stereospecific. However, both DFMO enantiomers suppressed ODC activity in a time- ...
متن کاملEffect on prostatic growth of 2-difluoromethylornithine, an effective inhibitor of ornithine decarboxylase.
2-Difluoromethylornithine (DFMO), an enzyme-activated irreversible inhibitor of ornithine decarboxylase, causes marked changes in the polyamine metabolism of ventral prostate when given to adult rats in drinking water (20 g/l) for 3 consecutive days. A 90% inhibition of ornithine decarboxylase activity is accompanied by approx. 80% decreases of the concentrations of putrescine and spermidine an...
متن کاملInhibition of ornithine decarboxylase with 2-difluoromethylornithine: reduced incidence of dimethylhydrazine-induced colon tumors in mice.
2-Difluoromethylornithine (DFMO) was administered to 1,2-dimethylhydrazine (DMH)-treated mice to reduce colonic polyamine levels and mucosal hyperplasia. Mice received 1% DFMO in drinking water throughout the experiment and were given injections of DMH (20 mg/kg) weekly for 28 weeks. DFMO inactivated 93% of colonic ornithine decarboxylase activity. Although DMH treatment did not induce colonic ...
متن کاملOverproduction of stable ornithine decarboxylase and antizyme in the difluoromethylornithine-resistant cell line DH23b.
DH23b cells, a variant of the HTC line selected for their resistance to difluoromethylornithine, exhibit defective feedback regulation of ornithine decarboxylase (ODC) stability and polyamine transport, and accumulate ODC protein to > 1000 times normal concentrations. The components of the polyamine feedback regulation system have been examined in an attempt to understand these unusual response...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2410305